Purification and Characterization of a-Carbonic Anhydrase II from Sheep Liver and Examining the Inhibition Effect of Kanamycin on Enzyme Activity Koyun Karaciğerinden a-Karbonik Anhidraz
نویسندگان
چکیده
Koyun karbonik anhidraz-II (E.C: 4.2.1.1) enzimi koyun karaciğerinden saflaştırıldı ve bazı karakteristik özellikleri araştırıldı. Enzim 4000 EU/mg protein spesifik aktivitesi ve % 38.6 verim ile yaklaşık olarak 43,1 kat saflaştırıldı. Enzim için optimum pH, optimum sıcaklık, optimum iyonik şiddet ve stabil pH sırasıyla 7.5, 40oC, 10 mM ve 8.5 olarak belirlendi. Moleküler ağırlığı sodyum dodesil sülfat poliakrilamid jel elektroforezi ile 29 kDa olarak bulundu. Kanamisinin enzim aktivitesi üzerine in vitro inhibitör etkisi incelendi.
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